The biochemistry Analytical laboratory facility is available for use after booking the facility by contact through Email or Telephone - Refer Contact details
Facility
HPLC : HP- HPLC 1101 – Agilent Technologies With UV and Fluorescent detector.
Shimadzu HPLC : Prominence
With the following detectors
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UV |
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Fluorescent |
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Diode array |
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Electrocheical |
Pre column and Post column derivatization facility
Clinical /Biological specimen Analysis
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Amino acid analysis in plasma and urine |
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Amino acid profile in other biological specimen.. (free amino acid / protein composition in hydrolysate**) |
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Vitamin A, E, C and D |
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Serotonin and other small molecules/metabolites |
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Lidocaine |
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Others: Other molecules will be scrutinized before acceptance and will include standardization. The standards are to be provided.
NABL accredited test – as per ISO15189: 2007 standards
** - Protocol for protein hydrolysis and sample preparation a can be obtained on request at the contact address.. |
Amino acid analysis in plasma and urine *
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Amino acid analysis in plasma and urine |
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Amino acid profile in other biological specimen.. (free amino acid / protein composition in hydrolysate**) |
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Vitamin A, E, C and D |
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Serotonin and other small molecules/metabolites |
| |
Lidocaine |
| |
Others: Other molecules will be scrutinized before acceptance and will include standardization. The standards are to be provided.
NABL accredited test – as per ISO15189: 2007 standards
** - Protocol for protein hydrolysis and sample preparation a can be obtained on request at the contact address.. |
List of Amino acid in the profile
Profile 1:
Ser, His, Gly, Ala, Arg, Tyr, Thr, Val, Met, Phe, Leu, Lys, Ile, His.
Profile 2 :
Ser, Tau, OHPro, Thr,Glu, ,Pro, Ala, Val, Met,
ILe, Leu, Tyr, Phe, His, Lys, Arg, Orn.
| HPLC 1101- Agilent |
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HPLC- Shimadzu Prominence |
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Atomic Absorption Spectrophotometer
A700 – Perkin Elmer With Flame and Graphite Furnace
Trace Element Analysis in biological specimen
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Copper |
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Zinc |
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Iron |
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Chromium |
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Selenium |
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Arsenic |
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Mercury |
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Cadmium |
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Lead |
Atomic Absorption spectrophotometer A700, with flame and graphite furnace
Our Publications based on HPLC/AAS analysis:
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Analysis of free and protein amino acids in germinating seedlings of soybean by HPLC. Insight XIX (1): 33-38;2001. |
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Tear ascorbic acid levels and the total antioxidant status in contact lens wearers. Indian J. Ophthalmol: 2009;57:289-92.
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Diffusion of lidocaine buffered to an optimal pH across the endotracheal tube cuff – an in vitro studies Indian J. Anaesth. 2008;52(5):536-40. |
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Hcy. levels in vitreous of PDR and RRD-its modulating role on LOX activity . Invest Ophthalmol Vis Sci. 2009 Apr 15.
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Hhcys, as well as low methionine stress are risk factors for CRVO. IOVS. 2007;48:1441-6. |
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Estimation of Taurine and its relevance in retina. Insight 2007;XXV(1):6-9 |
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Plasma Hcy and Total Thiol Content in patients with Exudative ARMD. Eye 2006;20:203–7
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DBS for vitamin A analysis – a field friendly method. Insight 2005;XXIII(1):20-24. |
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Involvement of oxidative and nitrosative stress in promoting retinal vacuities in patients with Eales’ disease. Clinical Biochemistry 2003;36(5):377-85. |
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Possible role of polyamines in gyrate atrophy. Indian J Ophthalmol 2000;48:37-43.
Antioxidant Vitamins E, C and A. 1999;Insight XVII(3):59. |
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Oral supplementation of Zinc promotes erythrocyte SOD activity in chronic cigarette smokers - report on a pilot clinic trial. Indian J Pharmacol 33(3):224;2001. |
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Purification and characterization of an 88 kDa novel protein from patients with Eales disease. Exp. Eye Res. 2001;73:547-55. |
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Increased level of Blood Cadmium and decreased Erythrocyte SOD activity in chronic tobacco chewers. Insight XVI (2):39-41;1998. |
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First report of congenital or infantile cataract in deranged metabolism with released xylose. Br J Ophthalmol 81:319-323;1997.
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Smoking of beedies and cataract: cadmium and vitamin C in the lens and blood. Br J Ophthalmol 79: 202-206; 1995. |
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Purification and characterization of a new enzyme dipeptidase from human lens. Exp. Eye. Res 62:221-229;1996 |
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Increased level of Blood Cadmium and decreased Erythrocyte SOD activity in chronic tobacco chewers. Insight XVI (2):39-41;1998. |
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First report of congenital or infantile cataract in deranged metabolism with released xylose. Br J Ophthalmol 81:319-323;1997. |
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Smoking of beedies and cataract: cadmium and vitamin C in the lens and blood. Br J Ophthalmol 79: 202-206; 1995. |
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Purification and characterization of a new enzyme dipeptidase from human lens. Exp. Eye. Res 62:221-229;1996 |
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HPLC method for simultaneous determination of ampicillin and sulbactam in biological samples. Indian J Pharmacol 27:189-192;1995. |
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Decrease in glycation of lens proteins by lysine and glycine by scavenging of glucose and possible mitigation of cataractogenesis. Exp. Eye Res. 1993;57:623-8. |
Contact Details :
Dr. K.N. Sulochana, Prof & Head
Department of Biochemistry and Cell biology
5th floor, New Research block, Vision Research Foundation
New No. 41, College Road, Nungambakkam, Chennai 600 006
Tel: 28271616 Extn. 1341 / 1304, Fax: 28254180
Email: drkns@snmail.org / drak@snmail.org
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