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Sankara Nethralaya
Sankara Nethralaya
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Back to Biochemistry
Department of Biochemistry and Cell Biology
 
Cell Biology
Cultured Primary Bovine RPE cells
Primary BRPE cells
Dividing epithelial cells
Cells after 1st passage
Actin
Vimentin
Cytokeratin 8.13
ARPE – 19 cells (ATCC)
ARPE 19 confluent layer
Cytokeratin 8.13 Positive
Primary culture of Bovine retinal capillary endothelial cells (BREC) cells and the markers of BREC cells
Confluent BREC cells after isolation with CD31 coated dynabeads,
Factor VIII positivity in BREC cells green fluorescence cytoplasmic,
VE cadherin positivity in BREC cells green fluorescence membrane
Primary culture of Bovine retinal capillay pericytes (BRP )cells and the markers
Confluent BRP cells on day 12,
NG2 positive BRP cells green fluorescence membrane positivity,
Actin filaments stained brown shown by immunohistochemistry in BRP cells.
Cultured Primary Bovine RPE cells
Primary BRPE cells Dividing epithelial cells
 
Cells after 1st passage Actin
 
Vimentin Cytokeratin 8.13
 
Cytokeratin – WB , showing a 46 KD band at passages 1-5 as indicated by the lanes 1-5
FITC conjugated Ab /Counter stain by Evans blue
ARPE – 19 cells (ATCC)
ARPE 19 confluent layer Cytokeratin 8.13 Positive
 
Cytplasmic postivity of FITC labelled (green) cytokeratin 8.13 characteristic of RPE
Figure 2 : Primary culture of Bovine retinal capillary endothelial cells (BREC) cells and the markers of BREC cells.
A
 
B   C
A: Confluent BREC cells after isolation with CD31 coated dynabeads,
B: Factor VIII positivity in BREC cells green fluorescence cytoplasmic,
C: VE cadherin positivity in BREC cells green fluorescence membrane
Figure 3 : Primary culture of Bovine retinal capillay pericytes (BRP )cells and the markers
A
 
B   C
A: Confluent BRP cells on day 12,
B: NG2 positive BRP cells green fluorescence membrane positivity ,
C: Actin filaments stained brown shown by immunohistochemistry in BRP cells.
A
 
B   C
Fig. 1: 3T3-L1 fibroblast cell line passage 11: Preadipocyte.
Fig. 2: Differentiated adipocyte cell line with oil droplets (10X).
Fig. 3: Oil red O stain for lipid droplets in adipocytes (20 X)
 
CHO-K1
 
Human choroidal endothelial cells   Human retinal endothelial cells
 
Human retinal pigment epithelial cells   Human umblical vein endothelial cells
 
Pheochromocytoma cells 1   Pheochromocytoma cells