HPLC analysis
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Aminoacids
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Vitamins A & E |
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Vitamin C |
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Tear antioxidants |
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Homocysteine |
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Drugs – Ampicillin and lidocaine |
Enzyme analysis
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Matrix metallo proteases
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Lysyl oxidase |
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superoxide dismurtase |
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Arylesterase |
Trace element analysis
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Cadmium
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Zinc |
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Selenium |
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Copper etc |
Proteomics
Clinical Proteomics in the discovery of protein peptide biomarkers characteristic of the clinical pathology
Facility Available
Two Dimensional gel Electrophoresis (BIORAD) using Immobilized pH Gradient (IPG) strips
Peptide spot analysis using
PD Quest 8.0.1.
Lipidomics
Analysis of plasma and vitreous omega fatty acids and its derivatives by LC-MS
Established primary cells from Bovine and Human
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Retinal pigment epithelial cells
Retinal endothelial cells
Retinal pericyte and Adipocytes |
Signal transduction
Insulin signal transduction
Extracellular matrix biology
Apoptosi
| Matrigel tube formation assay |
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Cell apoptosis assay |
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| Immunolocalization |
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Cell migration assay |
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Publications in Metabolic Diseases
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Pregnancy-exaggerated galactosemia and congenital cataracts. Indian J Pediatr 1998;65:919-24
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Smoking of beedies and cataract: cadmium and vitamin C in the lens and blood. Br J Ophthalmol 79: 202-206; 1995 |
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First report of congenital or infantile cataract in deranged metabolism with released xylose. Br J Ophthalmol 81:319-323;1997. |
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Possible role of polyamines in gyrate atrophy. Indian J Ophthalmol 2000;48:37-43. |
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Insulin Receptor Tyrosine Kinase Activity in monocytes of Type 2 Diabetes Mellitus Patients receiving oral L-Lysine. Indian Journal Biochemistry Biophysics. 2001;38: 331-334. |
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Hhcys, as well as low methionine stress are risk factors for CRVO. IOVS. 2007;48:1441-6. |
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Plasma Hcy and Total Thiol Content in patients with Exudative ARMD. Eye 2006;20:203–207 |
Flow cytometry analysis of GLUT4
Western blot for PEDF,RAGE, VEGF, GLUT, AKT, PI3K and many others are done
Liquid scintillation system
mRNA expression of VEGF in retinal endothelial cells
Angiogenesis in diseases
A common denominator in many diseases.
Focus
Understanding pathways of growth factors and inhibitor
Therapeutic angiogenesis
Preclinical and Clinical studies
Bioassay for analysis of in vivo and
in vitro angiogenesis
Molecular mechanisms of the drug
Drug assay and Pharmaco dynamics
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