Biochemistry Research Updates

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Research Updates

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	Ocular Tissue distribution of Lysyl oxidase enzyme LOX- (IOVS 2008)
	LOX RT-PCR in ARPE cells exposed to high glucose after 24 hrs and 72 hrs
	IOVS 2009
	Increased GLUT 4 expression in the presence of Amino acid mixture – FACS analysis
	Tear Proteomics – 2D electrophoresis
	VEGF expression in Bovine Retinal capillary Endothelial cells

Ocular Tissue distribution of Lysyl oxidase enzyme LOX- (IOVS 2008)

LOX RT-PCR in ARPE cells exposed to high glucose after 24 hrs and 72 hrs

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156 bp

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Lane 1 : Negative control

Lane 2 : 24 hrs –control (5.5 mM Glucose)

Lane 3 : 24 hrs – 10 mM Glucose

Lane 4 : 24 hrs - 20 mM Glucose

Lane 5 : 24 hrs - 30 mM Glucose

Lane 6 : 72 hrs –control (5.5mM Glucose)

Lane 7 : 72 hrs – 10 mM Glucose

Lane 8 : 72 hrs - 20 mM Glucose

Lane 9 : 72 hrs - 30 mM Glucose

Lane 10 : 1000 bp marker

LOX mRNA expression (156bp) was seen in all conditions with a 60% and 40% lowered expression in 20 mM and 30 mM of 72 hours (Lane 8 and 9).

IOVS 2009

Distribution of Homocysteine in PDR, RRD and Donor eye ball control

Increased GLUT 4 expression in the presence of Amino acid mixture – FACS analysis

Log VEGF/PEDF
VEGF/PEDF ratio in ED / MH / PDR.

Tear Proteomics – 2D electrophoresis

VEGF expression in Bovine Retinal capillary Endothelial cells

GAPDH

Lane 1 : Negative control

Lane 2 : control

Lane 3 : AGE

Lane 4 : AGE + 0.5mM amino acid

Lane 5 : AGE + 2.5mM amino acid

Lane 6: mol wt

VEGF

mRNA expression of the house keeping gene GAPDH and the angiogenic marker VEGF in bovine retinal capillary Endotheila cells. A: GAPDH of BREC cells, B: VEGF of BREC cells, VEGF mRNA expression aftertreatment with 2.5mM glycine & glutamic acid.

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