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Sankara Nethralaya
Sankara Nethralaya
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Central Research Instrumentation Facility
AGILENT 2100 BIOANALYSER
Faculty incharge-Dr.K.Lily Therese / Dr.Sripriya
Agilent 2100 Bioanalyser is a Micro fluidics- chip based technology for the analysis of Biological samples (DNA, RNA, Protein and Cell analysis) and it is automated, fast analysis and high quality digital data within 30 minutes.
To book Instruments Call : 28271616 (1325), 28272727
SPECIAL FEATURES
Faster than gels (digital data in 30 minutes for quality and quantity of up to 12 samples)
Reproducible and complete digital data
Easy to use and share
Quantification of each individual fragment per sample offers purity results
2-color analysis of fluorescently stained cells
Compliance Services allow work within regulated environments (e.g. QA/QC labs)
2100 Expert Software for easy digital handling and storage of all bioanalyzer data
2100 Security Pack Software providing tools for 21CFR part 11 compliance

VARIOUS AREAS OF APPLICATIONS

DNA Analysis (sizing and quantitation)

Gene Expression Analysis
Detection of Genetically modified Organisms (GMO)
Restriction digest and PCR product Analysis
Oncology, Diagnostic Research and Forensic Testing
RNA Analysis
Analysis of total RNA and mRNA
RNA Integrity Number (RIN) - Standardization of RNA Quality Control
Analysis of Small RNA molecules e.g., miRNA, siRNA, t-RNA
Analysis of Cy5 labeled samples and cRNA fragmentation
Protein Analysis
Protein Expression and Purification
His –tag protein purification
Protein sizing and quantification in ng range
Protein detection in pg range: High sensitivity and QC/QA application
Cell Fluorescence Analysis
Protein expression Monitoring
Transfection Efficiency Monitoring (Expression of GFP)
Apoptotic Detection and Gene monitoring in Cell Cultur
Cell Fluorescence Analysis
Protein expression Monitoring
Transfection Efficiency Monitoring (Expression of GFP)
Apoptotic Detection and Gene monitoring in Cell Cultur
Protein detection in pg range: High sensitivity and QC/QA application
Cell Fluorescence Analysis
Protein expression Monitoring
Transfection Efficiency Monitoring (Expression of GFP)
Apoptotic Detection and Gene monitoring in Cell Cultur