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Sankara Nethralaya
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Central Research Instrumentation Facility
ZEISS AXIO OBSERVER FLUORESCENT MICROSCOPE
FACULTY INCHARGE: Dr.KRISHNAKUMAR / Dr.NIVEDHITA CHATTERJEE

Zeiss Axio Observer fully automated research class microscope complete with linear encoded stage capable of Phase Contrast, DIC, BF and Fluorescence Illumination utilizing Mercury arc lamps.

To book Instruments Call : 28271616 (1325), 28272727
Features:
Zeiss Axiovert 200M motorized inverted microscope with 10X conventional and 20X DIC objectives and 40X, 63X, and 100X DIC oil-immersion objectives.
Axio Observer microscopes are inverted light microscopes for universal use. They are used primarily for examining cell and tissue cultures and sediments in culture flasks, Petri dishes and micro titer plates under transmitted and reflected light.Axio Observer microscopes can be used for bright field, phase contrast, differential interference contrast, and PlasDIC contrast transmitted light techniques and epifluorescence techniques.
Axio Observer microscopes form the basis for microscopy work on living cells.
Mercury arc lamps are used for fluorescence microscopy instead of lasers. This gives a wide choice of fluorophores, and is not limited to 2 or 3 specific excitation lines, as with lasers. Also, Hg illumination allows the excitation of UV and short wavelength blues dye, such as DAPI and the Hoechst nuclear dyes.
The robust stand allows the attachment of a variety of tools (micromanipulation), various light sources and incubation components.
The microscope's inverted design, LD condensers and the use of fixed stages means that there is sample space for specimens and mounting frames. This allows experiments which would not be possible with upright microscopes to be performed.
The design allows the simple attachment of cameras, lasers, custom stages, etc.

Typical fields of applications:

Examination of human blood and tissue samples, observation of intracellular processes in living cell cultures, cell/cell interactions, motility, growth, measurement of potentials, drug detection,
microinjection, IVF (in-vitro fertilization), toxicity studies, patch-clamp techniques, ion measurements,digital recordings, time lapse studies with automated processes, z-sectioning, deconvolution, visualization of molecular structures, Fura (Ca measurement), GFP, optical tweezers and scissors, single molecule detection, TIRF.

Includes:

Stand; Binocular head; Fixed stage plate; Condenser; Neutral density and conversion filters; Appropriate filter sets for phase contrast and fluorescence; Objectives; Eyepieces; Illumination system; Reflectors, Sliders, Lamps and housing for bright field, phase contrast and epi-fluorescence illumination; Power supply and 110V power cable; Dust cover.