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  Retinoblastoma Pathology Stem cellNanotechnologyGlial Biology
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  Nanobiotechnology Lab

Corneal stem cell Research

Expression of corneal stem cell/differentiate markers, signal transduction pathways involved in the growth on various scaffolds using RT-PCR and microarray analysis.

Retinal stem cell Research

Isolation of the Retinal stem cells from the ciliary pigmented epithelial cells and iris pigmented epithelial cells in vitro and stem cell/ differentiate marker expression by RT- PCR and microarray analysis. The functional aspects of the isolated retinal stem/progenitor cells will be studied by live cell imaging.

Proteomics profiling for retinoblastoma and analysis for the expression of intracellular proteins between non-invasive and invasive tumors and tumor cell line.

The expression of multidrug resistant proteins-MRP (P-gp, MRP1 and LRP), effect of curcumin on the expression of MRP and the antitumor effect of curcumin, effect of Fatty acid synthase (FASN) inhibitors: Cerulenin, Triclosan and Orlistat, the apoptotic genes in cells treated with FASN inhibitors and analysis of lipid components like ceramide in treated on Y79 retinoblastoma cell line

We are using RNA interference against specific proteins to study downstream pathways. siRNA is being used to study proteins involved in cancer for possible targeting against metastatic cells. We are also in the process of using nanotechnology as a delivery tool for siRNA.

Calcium wave dynamics in cocultures - Calcium being one of the fundamental second messengers, we are looking at the dynamics of calcium wave propagation in neuron-glia coculture and the signaling pathways that are involved. This has particular relevance since calcium patterns change during certain pathologic conditions.

Neuroprotective role of Mueller glia – Being the predominant glia in the retina, Mueller glia are involved in all retinal disorders to some extent and play seminal role in the normal retina in the adult as well as the developing stage of the tissue. We study the beneficial effects Mueller cells have in protecting retinal neurons by RT-PCR, ELISA, microarray and standard biochemical and cell biology techniques. We are also using RNA knockdown to study neuroprotective pathways in Mueller glia.